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cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 718 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cyclin+d1+antibody/Cyclin+D1+XP+Rabbit+mAb/pm42883230-257-39-41
    Average 96 stars, based on 718 article reviews
    cyclin d1 - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: MIAT promotes tumor-infiltrating CD8+ T-cell exhaustion and malignant progression of renal cell carcinoma via activating JAK3/STAT3 pathway
    Article Snippet: .. After being blocked with 5% fat-free milk, the PVDF membranes were incubated overnight at 4°C with the following primary antibodies: anti-JAK3 antibody (Catalog number: ab45141, 1:1000) and anti-MYC antibody (Catalog number: ab32072, 1:1000) purchased from Abcam (Cambridge, UK), anti-STAT3 antibody (Catalog number: 12640, 1:1000), anti-p-STAT3 antibody (Catalog number: 9145, 1:2000), anti-ETS1 antibody (Catalog number: 14069, 1:2000) and anti-cyclin D1 antibody (Catalog number: 55506, 1:1000) purchased from Cell Signaling Technology (Massachusetts, USA), anti-PD-L1 antibody (Catalog number: 66 248–1-Ig, 1:2000) obtained from Proteintech (Wuhan, China). ..

    Article Title: MIAT promotes tumor-infiltrating CD8 + T-cell exhaustion and malignant progression of renal cell carcinoma via activating JAK3/STAT3 pathway.
    Article Snippet: .. After being blocked with 5% fat- free milk, the PVDF membranes were incubated overnight at 4°C with the following primary antibodies: anti- JAK3 antibody (Catalog number: ab45141, 1:1000) and anti- MYC antibody (Catalog number: ab32072, 1:1000) purchased from Abcam (Cambridge, UK), anti- STAT3 antibody (Catalog number: 12640, 1:1000), anti- p- STAT3 antibody (Catalog number: 9145, 1:2000), anti- ETS1 antibody (Catalog number: 14069, 1:2000) and anti- cyclin D1 antibody (Catalog number: 55506, 1:1000) purchased from Cell Signaling Technology (Massachusetts, USA), anti- PD- L1 antibody (Catalog number: 66 248–1- Ig, 1:2000) obtained from Proteintech (Wuhan, China). ..

    other:

    Article Title: Mechanism of D-type cyclins recognition by the AMBRA1 E3 ligase receptor
    Article Snippet: Anti-AMBRA1 antibody was used at 1:1000 dilution (CST, 24907s), anti-Phospho-Histone H2A.X (Ser139) antibody was used at 1:1000 dilution (CST, 2577s), Fab2 Fragment (Alexa Fluor 647 Conjugate) was used at 1:1000 dilution (CST, 4414s), anti-cyclin D1 antibody was used at 1:1000 dilution (CST, 2922S and Santa Cruz, sc-20044), anti-cyclin D2 antibody was used at 1:1000 dilution (Proteintech, 10934-1-AP), anti-cyclin D3 antibody was used at 1:1000 dilution (Proteintech, 26755-1-AP), anti-Phospho-cyclin D1 (Thr286) antibody was used at 1:2000 dilution (CST, 3300T), anti-Phospho-cyclin D2 (Thr280) antibody was used at 1:2000 dilution (Abcam, AB230818), anti-Phospho-cyclin D3 (Thr283) antibody was used at 1:2000 dilution (Affinity, AF3251), anti-β-Tubulin antibody was used at 1:1000 dilution (Novus, NB600-936), anti-MBP tag Mouse Monoclonal antibody was used at 1:1000 dilution (GenScript, A00190), GST tag Mouse Monoclonal antibody was used at 1:1000 dilution (STARTER, S-372-19), the secondary antibodies conjugated to horseradish peroxidase (HRP), Goat anti-Mouse IgG was used at 1:2000 dilution (CWBIO, CW0102), Goat anti-Rabbit IgG was used at 1:2000 dilution (CWBIO, CW0103).

    Article Title: A novel taxane SB-T-101141 triggers a noncanonical ferroptosis to overcome Paclitaxel resistance of breast cancer via iron homeostasis-related KHSRP
    Article Snippet: Anti-β-Tubulin antibody (catalog no. 2146), anti-eIF2α antibody (catalog no. 5324), anti-phospho-eIF2α antibody (catalog no. 3398), Phospho-MAPK Family Antibody Sampler Kit (catalog no. 9910), MAPK Family Antibody Sampler Kit (catalog no. 9926), anti-GAPDH antibody (catalog no. 2118), anti-PARP antibody (catalog no. 9542), anti-Caspase-7 antibody (catalog no. 9492), anti-Cyclin B1 antibody (catalog no. 12231), anti-Cdc25c antibody (catalog no. 4688), anti-Cyclin D1 antibody (catalog no. 55506), anti-Cyclin D3 antibody (catalog no. 2936), anti-CDK4 antibody (catalog no. 12790), anti-CDK6 antibody (catalog no. 13331), anti-CDK2 antibody (catalog no. 2546), anti-P21 antibody (catalog no. 2947), anti-P27 antibody (catalog no. 3686), anti-mouse IgG, HRP-linked antibody (catalog no. 7076) and anti-rabbit IgG and HRP-linked antibody (catalog no. 7074) were obtained from Cell Signaling Technology.



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    Changes in the hypertrophic chondrocytes of 13del-MCDS mice. a Immunofluorescent staining of CollagenX (ColX) was performed on the proximal tibia growth plate of P10 mice. ColX proteins can be detected in the ECM of +/+ and M/+ mice, while some or most are accumulated inside the cells in M/+ and M/M mice, respectively (white arrows). b In situ hybridization of Bip mRNA and immunohistochemistry staining of Xbp1s on the proximal tibia growth plate of 10-day-old mice. c In situ hybridization of Col2a1 , Ppr , and Ihh mRNA on tibia paraffin sections on the proximal tibia growth plate of 10-day-old mice. d BrdU staining and <t>Cyclin</t> <t>D1</t> immunohistochemistry staining on the proximal tibia growth plate of 4-week-old mice. Higher magnifications of the respective boxed regions in the proliferative zone and hypertrophic zone are shown (HZ, hypertrophic zone; UHZ, upper hypertrophic zone; MHZ, middle hypertrophic zone; LHZ, lower hypertrophic zone). e In situ hybridization of Col2a1 , Col10a1 and Ppr mRNA on proximal tibia growth plate of 4-week-old mice, showing two consecutive Col10a1 positive hypertrophic zones and two rounds (R1 and R2) chondrocyte hypertrophy. Quantification of positive cells for BrdU in ( f ) and Cyclin D1 in ( g ) ( n = 3). h In situ hybridization of Mmp13 mRNA on the proximal tibia growth plate of 10-day-old and 4-week-old mice
    Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1  (Cell Signaling Technology Inc)
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    Changes in the hypertrophic chondrocytes of 13del-MCDS mice. a Immunofluorescent staining of CollagenX (ColX) was performed on the proximal tibia growth plate of P10 mice. ColX proteins can be detected in the ECM of +/+ and M/+ mice, while some or most are accumulated inside the cells in M/+ and M/M mice, respectively (white arrows). b In situ hybridization of Bip mRNA and immunohistochemistry staining of Xbp1s on the proximal tibia growth plate of 10-day-old mice. c In situ hybridization of Col2a1 , Ppr , and Ihh mRNA on tibia paraffin sections on the proximal tibia growth plate of 10-day-old mice. d BrdU staining and <t>Cyclin</t> <t>D1</t> immunohistochemistry staining on the proximal tibia growth plate of 4-week-old mice. Higher magnifications of the respective boxed regions in the proliferative zone and hypertrophic zone are shown (HZ, hypertrophic zone; UHZ, upper hypertrophic zone; MHZ, middle hypertrophic zone; LHZ, lower hypertrophic zone). e In situ hybridization of Col2a1 , Col10a1 and Ppr mRNA on proximal tibia growth plate of 4-week-old mice, showing two consecutive Col10a1 positive hypertrophic zones and two rounds (R1 and R2) chondrocyte hypertrophy. Quantification of positive cells for BrdU in ( f ) and Cyclin D1 in ( g ) ( n = 3). h In situ hybridization of Mmp13 mRNA on the proximal tibia growth plate of 10-day-old and 4-week-old mice
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    Changes in the hypertrophic chondrocytes of 13del-MCDS mice. a Immunofluorescent staining of CollagenX (ColX) was performed on the proximal tibia growth plate of P10 mice. ColX proteins can be detected in the ECM of +/+ and M/+ mice, while some or most are accumulated inside the cells in M/+ and M/M mice, respectively (white arrows). b In situ hybridization of Bip mRNA and immunohistochemistry staining of Xbp1s on the proximal tibia growth plate of 10-day-old mice. c In situ hybridization of Col2a1 , Ppr , and Ihh mRNA on tibia paraffin sections on the proximal tibia growth plate of 10-day-old mice. d BrdU staining and <t>Cyclin</t> <t>D1</t> immunohistochemistry staining on the proximal tibia growth plate of 4-week-old mice. Higher magnifications of the respective boxed regions in the proliferative zone and hypertrophic zone are shown (HZ, hypertrophic zone; UHZ, upper hypertrophic zone; MHZ, middle hypertrophic zone; LHZ, lower hypertrophic zone). e In situ hybridization of Col2a1 , Col10a1 and Ppr mRNA on proximal tibia growth plate of 4-week-old mice, showing two consecutive Col10a1 positive hypertrophic zones and two rounds (R1 and R2) chondrocyte hypertrophy. Quantification of positive cells for BrdU in ( f ) and Cyclin D1 in ( g ) ( n = 3). h In situ hybridization of Mmp13 mRNA on the proximal tibia growth plate of 10-day-old and 4-week-old mice
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    Changes in the hypertrophic chondrocytes of 13del-MCDS mice. a Immunofluorescent staining of CollagenX (ColX) was performed on the proximal tibia growth plate of P10 mice. ColX proteins can be detected in the ECM of +/+ and M/+ mice, while some or most are accumulated inside the cells in M/+ and M/M mice, respectively (white arrows). b In situ hybridization of Bip mRNA and immunohistochemistry staining of Xbp1s on the proximal tibia growth plate of 10-day-old mice. c In situ hybridization of Col2a1 , Ppr , and Ihh mRNA on tibia paraffin sections on the proximal tibia growth plate of 10-day-old mice. d BrdU staining and <t>Cyclin</t> <t>D1</t> immunohistochemistry staining on the proximal tibia growth plate of 4-week-old mice. Higher magnifications of the respective boxed regions in the proliferative zone and hypertrophic zone are shown (HZ, hypertrophic zone; UHZ, upper hypertrophic zone; MHZ, middle hypertrophic zone; LHZ, lower hypertrophic zone). e In situ hybridization of Col2a1 , Col10a1 and Ppr mRNA on proximal tibia growth plate of 4-week-old mice, showing two consecutive Col10a1 positive hypertrophic zones and two rounds (R1 and R2) chondrocyte hypertrophy. Quantification of positive cells for BrdU in ( f ) and Cyclin D1 in ( g ) ( n = 3). h In situ hybridization of Mmp13 mRNA on the proximal tibia growth plate of 10-day-old and 4-week-old mice
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    Changes in the hypertrophic chondrocytes of 13del-MCDS mice. a Immunofluorescent staining of CollagenX (ColX) was performed on the proximal tibia growth plate of P10 mice. ColX proteins can be detected in the ECM of +/+ and M/+ mice, while some or most are accumulated inside the cells in M/+ and M/M mice, respectively (white arrows). b In situ hybridization of Bip mRNA and immunohistochemistry staining of Xbp1s on the proximal tibia growth plate of 10-day-old mice. c In situ hybridization of Col2a1 , Ppr , and Ihh mRNA on tibia paraffin sections on the proximal tibia growth plate of 10-day-old mice. d BrdU staining and <t>Cyclin</t> <t>D1</t> immunohistochemistry staining on the proximal tibia growth plate of 4-week-old mice. Higher magnifications of the respective boxed regions in the proliferative zone and hypertrophic zone are shown (HZ, hypertrophic zone; UHZ, upper hypertrophic zone; MHZ, middle hypertrophic zone; LHZ, lower hypertrophic zone). e In situ hybridization of Col2a1 , Col10a1 and Ppr mRNA on proximal tibia growth plate of 4-week-old mice, showing two consecutive Col10a1 positive hypertrophic zones and two rounds (R1 and R2) chondrocyte hypertrophy. Quantification of positive cells for BrdU in ( f ) and Cyclin D1 in ( g ) ( n = 3). h In situ hybridization of Mmp13 mRNA on the proximal tibia growth plate of 10-day-old and 4-week-old mice
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    Changes in the hypertrophic chondrocytes of 13del-MCDS mice. a Immunofluorescent staining of CollagenX (ColX) was performed on the proximal tibia growth plate of P10 mice. ColX proteins can be detected in the ECM of +/+ and M/+ mice, while some or most are accumulated inside the cells in M/+ and M/M mice, respectively (white arrows). b In situ hybridization of Bip mRNA and immunohistochemistry staining of Xbp1s on the proximal tibia growth plate of 10-day-old mice. c In situ hybridization of Col2a1 , Ppr , and Ihh mRNA on tibia paraffin sections on the proximal tibia growth plate of 10-day-old mice. d BrdU staining and Cyclin D1 immunohistochemistry staining on the proximal tibia growth plate of 4-week-old mice. Higher magnifications of the respective boxed regions in the proliferative zone and hypertrophic zone are shown (HZ, hypertrophic zone; UHZ, upper hypertrophic zone; MHZ, middle hypertrophic zone; LHZ, lower hypertrophic zone). e In situ hybridization of Col2a1 , Col10a1 and Ppr mRNA on proximal tibia growth plate of 4-week-old mice, showing two consecutive Col10a1 positive hypertrophic zones and two rounds (R1 and R2) chondrocyte hypertrophy. Quantification of positive cells for BrdU in ( f ) and Cyclin D1 in ( g ) ( n = 3). h In situ hybridization of Mmp13 mRNA on the proximal tibia growth plate of 10-day-old and 4-week-old mice

    Journal: Bone Research

    Article Title: Integrated stress signaling pathways promote adaptive reprogramming for chondrocyte survival in Metaphyseal Chondrodysplasia Type Schmid

    doi: 10.1038/s41413-026-00587-5

    Figure Lengend Snippet: Changes in the hypertrophic chondrocytes of 13del-MCDS mice. a Immunofluorescent staining of CollagenX (ColX) was performed on the proximal tibia growth plate of P10 mice. ColX proteins can be detected in the ECM of +/+ and M/+ mice, while some or most are accumulated inside the cells in M/+ and M/M mice, respectively (white arrows). b In situ hybridization of Bip mRNA and immunohistochemistry staining of Xbp1s on the proximal tibia growth plate of 10-day-old mice. c In situ hybridization of Col2a1 , Ppr , and Ihh mRNA on tibia paraffin sections on the proximal tibia growth plate of 10-day-old mice. d BrdU staining and Cyclin D1 immunohistochemistry staining on the proximal tibia growth plate of 4-week-old mice. Higher magnifications of the respective boxed regions in the proliferative zone and hypertrophic zone are shown (HZ, hypertrophic zone; UHZ, upper hypertrophic zone; MHZ, middle hypertrophic zone; LHZ, lower hypertrophic zone). e In situ hybridization of Col2a1 , Col10a1 and Ppr mRNA on proximal tibia growth plate of 4-week-old mice, showing two consecutive Col10a1 positive hypertrophic zones and two rounds (R1 and R2) chondrocyte hypertrophy. Quantification of positive cells for BrdU in ( f ) and Cyclin D1 in ( g ) ( n = 3). h In situ hybridization of Mmp13 mRNA on the proximal tibia growth plate of 10-day-old and 4-week-old mice

    Article Snippet: It was subsequently probed with an appropriately diluted primary antibody overnight at 4 o C. The next step was incubation with a horseradish peroxidase-conjugated secondary antibody (which recognized the primary antibody) for 30 min. Immunohistochemistry and immunofluorescence staining were performed using antibodies specific for Collagen X and 13del mutant Collagen X (Generated by our lab ), Cyclin D1 (Santa Cruz, Cat#sc-753), Xbp1s (BioLegend, Cat# 647502), CD31 (Abcam, Cat# ab28364), HIF1a (SANTA CRUZ BIOTECH, Cat# sc-10790), HIF2a (SANTA CRUZ BIOTECH, Cat# sc-28706), p53 (E-19) (SANTA CRUZ BIOTECH, Cat# sc-1315), Tap63 (NOVUS BIOLOGICALS, Cat# NBP3-11703), ΔNp63 (BioLegend, Cat# 619001), EDEM (Provided by Kumamoto University, Japan), iASPP (Sigma, Cat# A4605), ASPP2 (Provided by Dr. Xin Lu from University of Oxford), ATF4 (SANTA CRUZ BIOTECH, Cat# sc-200), p57Kip2 (SANTA CRUZ BIOTECH, Cat# sc-56341), EF5-Cy3 regular stain antibody and EF5-competed stain antibody (Provided by Dr. Cameron Koch from University of Pennsylvania Department of Radiation Oncology), Map-LC3B (MBL life science, Cat# PM036), CHOP (SANTA CRUZ BIOTECH, Cat# sc-166682), GADD34 (SANTA CRUZ BIOTECH, Cat# sc-794), ATF3 (SANTA CRUZ BIOTECH, Cat# sc-81189), 488 Goat Anti-Rabbit IgG (Life technologies, Cat# A-11008) were used.

    Techniques: Staining, In Situ Hybridization, Immunohistochemistry, BrdU Staining